Butterfly decline in the UK some new hypotheses by Dr Chris Barnes, Bangor Scientific and Educational Consultants. E-mail manager@bsec-wales.co.uk

First published on-line without full reference  list   22nd August 2016

 

Abstract

Some existing and brand new hypotheses of butterfly decline causation are raised and discussed.  Climate is shown to be a relatively weak factor with only summer temperatures being relevant in a linear regression study.  Neonics are also relevant  as are ghlyphosphates.   However, the strongest single factor found in UK butterfly(permanent) decline by linear correlation appears to be the increasing prevalence of white skies due to Aviation    cirrus type cloud or persisting contrails.  These disrupt light polarization essential for butterfly navigation, feeding and mating.    Transient variations in butterfly pollutions  may also arise  as a result of new radio frequency technologies.  These disrupt cryptochrome  blue light sensing and magnetic navigation and the butterfly immune response.  Bees and other insects and birds may be similarly affected by both white skies and RF.      The decline of birds is perhaps inevitable, whenever insects are in decline as they are integral parts of a food chain.  The fact that the decline of farmland birds is more than double that of woodland and sea birds suggests a problem with the farmland environment but the fact that all birds are in decline    suggests more than one reason for decline with a second or additional reasons extending beyond farmland ( i.e. simple pesticide use)  and by extrapolation there may perhaps  similarly be expected   to be more than one reason for the decline of pollinators and in this case butterflies, especially as their caterpillars form an essential part of bird food chains.    

UK butterflies appear to be in a state of severe decline for a number of reasons.   This work finds that the strongest reason for their decline (73% of current rates) is the increasing prevalence of cloudy skies, especially aviation cirrus here in the UK.  Secondary reasons   include the neonicotinoid family of pesticides and the orgnophosphate herbicide glyphosphate.   Pesticides and herbicides possibly account   for the other 30% or so of current decline.  Transient declines (between 10-30 % of the 1990 total numbers) may have also occurred with the introduction of various new radio frequency communication and broadcasting technologies. 

 

The following further work is crucial to confirming the above hypotheses and preliminary findings:

1.             Establish butterfly numbers underneath UK air-lanes compared with numbers well separated from the same. Flight radar evidence already suggests that the most overflown areas are those with most butterfly decline.  This explains why even thermophilic butterflies are declining in Germany when theoretically their numbers  should be improving in a warming climate.    

2.             Establish decline ( if any)  due to 4G communications technology which  ought to show in 2012-2017 data. 

3.             Fight for moratorium on the use of 'round up' and similar glyphosphate weed killer products, to re-assess their effect. 

 

 

Introduction

 

Butterflies, partly because of their very short life cycles, are fast becoming accepted as new and exciting environmental indicators (ref). Presently, however, the precise factors to which they are sensitive are neither well known nor quantified. This present paper  aims to demystify the situation somewhat.     Any individual with an eye on their  garden or the countryside in general will not be able to help but notice the dramatic decline in butterfly numbers in recent years.  This  has been documented by Defra for the period 1990-2012 see   https://www.gov.uk/government/uploads/system/uploads/attachment_data/file/389401/agindicator-de6-18dec14.pdf

 

The Defra report states that although numbers fluctuate from year to year, presumably as a result of climatic conditions, there has been a more significant decline since 2008.

 

Butterflies are, along with bees, essential pollinators.  Another Defra report describes the decline of pollinators in more detail   see https://www.gov.uk/government/uploads/system/uploads/attachment_data/file/493028/UKBI_2015_v3a.pdf

 

and   http://nurturing-nature.co.uk/wildlife-garden-videos/defras-report-2015-birds-pollinators-habitats-are-in-decline/

The above references show that between

1970-2012, 3/4 birds, butterflies, moths and mammals populations of priority species have declined and distribution dramatically dropped.

1980-2010, Butterfly populations have crashed. More than 1/2 of all pollinator species, e.g. bees, hoveflies, are less widespread and declines are more exaggerated in more recent years.

1970-2014 breeding birds: decline 20% woodland, farmland 54% birds, 2014 seabirds 27% lower 1986 levels.

 

 

The decline of birds is perhaps inevitable, whenever insects are in decline as they are integral parts of a food chain.

 

The fact that the decline of farmland birds is more than double that of woodland and sea birds suggests a problem with the farmland environment but the fact that all birds are in decline    suggests more than one reason for decline with a second or additional reasons extending beyond farmland  and by extrapolation there may perhaps  similarly be expected   to be more than one reason for the decline of pollinators and in this case butterflies, especially as their caterpillars form an essential part of bird food chains.    

 

 

Over the past decade,  neonicotinoids  ( so called neonics for short) have been shown to be associated with bee decline. There are indeed hundreds of papers, far too many to reference here   which discuss the link and the mechanisms.  Others have posed the question can neonics be harmful to butterflies ( refs).   

 

I will examine the evidence for this below.    

 

I have also very recently noticed that some species of butterfly here in North Wales are having severe difficulty in orienting themselves and in choosing flowers on which to settle.  Such difficulty in itself might be expected to lead to butterfly   decline because such difficulty might be expected to prevent mate selection, mating, foraging and feeding.      This has prompted me to explore if there are other potential mechanisms for decline besides chemical exposure.  I will discuss my findings below.   I have also noticed that butterflies are avoiding urban gardens and that Woodland species of butterflies are not so badly affected.  

 

Data

 

The data used in this study is essentially that of the Defra report and data has also been taken from the Met office website regarding UK Climate Anomaly at   http://www.metoffice.gov.uk/public/weather/climate-anomalies/#?tab=climateAnomalies

 

Methods, Results and Discussion 

 

1.      Climate

 

Defra has suggested that the random fluctuation of butterfly populations is most likely due to weather.   To test this hypothesis I constructed linear regression algorithms  of butterfly population versus temperature anomaly for the period 1990-2013.  I also examined the effect of springtime, summertime and autumn temperatures.    For the entire period the regression values were

 

Spring = .06

Summer = .53

 r=.47   DF=23  

 

   The two-tailed P value equals 0.0064

   By conventional criteria, this difference for the Summer result is considered to be very statistically significant.

 

 

Autumn =.14 

 

There is no statistical significance to the spring or autumn results.

 

 

On the basis that I have noticed significantly whiter skies in the UK and fewer butterflies  since about the year 2000, I decided to produce individual summertime regressions  for the period 1990-2000 and 2001-2013.

 

The resultant algorithms are:

 

1990-2013      % population = 68.4  + 8.6*DeltaT

 

1990-2000  % population = 82+ 11.7* Delta T

 

2001-2013   % population = 55.6 + 12.15* Delta T  R=.53

 

The regression factor was strongest for the latter period.

 

The fact that the regression factor weakens as more data is added is indicative of the involvement of multiple processes   for the causation of butterfly decline.

 

 

1.      Neonics

In seeking what these multiple processes might be, I decided to explore the part that neonics might, if any, be playing. 

 

 

I plotted the Defra data against year number and applied a best sinusoidal fit. It can be clearly seen that the plot is relatively flat until 1994 and flat again but lower after 2011.  The very dates when neonics were first used and when the present moratorium came into play.  I recently contacted DEFRA and the Welsh Assembly Government regarding the state of play  of Gwynedd's Butterflies in the present summer (2016) to enquire if the moratorium had come to an end.    The answer was that it had not.     I was surprised, however, to find out that emergency arrangements had been made to allow the spraying of some oil seed rape in the second half of the summer of 2015 ( ref) and was somewhat disappointed that they had not personally confirmed this.   

 

Neonicotinoids were first used extensively on UK farmland in 1994 and the present moratorium came in to force in 2011.    However, the data is far from a good fit and other factors may also be at play especially as other mathematical models show continued decline.

 

2.      Glyphosphate

 

 

 Glyphosphate is being more widely used on wheat than ever before and as a garden weed killer.( Refs)  It is more toxic to humans than its declared intention as a herbicide (refs). It is being found in significant concentrations in human urine ( refs), is an endocrine disruptor ( refs) and may be carcinogenic to humans ( refs). Yet by some madness it is still allowed as an agricultural and garden herbicide.        It also disrupts some insect communities by altering weeds distributions. (refs)  

 

I thus wondered if glyphosphate may be altering butterfly populations. I made the linear plot below.

 

 

 

 

 

I find a linear relationship between glyphospahte application and butterfly decline in recent years but with             what I thought was a fairly weak correlation, R=.55.  Further inspection, however, due to the available number of degrees of freedom shows it to be significant.

 

P Value Results

r=.55   DF=16   

   The two-tailed P value equals 0.0180
   By conventional criteria, this difference is considered to be statistically significant.

 

My guess is both habitat destruction and direct organophosphate toxicity may be responsible here. 

 

There has been a tremendous recent upsurge in TV weed killer advertising and local councils weed spray all urban verges.  My guess is this will drive most urban butterflies into more rural locations, possibly distorting butterfly counts which rely on the public at large.      

 

3.      White skies/the effect  of increased air travel on butterflies.

 

I have previously proposed a hypothesis wherein RF radiation with rotating magnetic vector may effect butterflies via cryptochrome.   I will further discuss the effect of the RF background on butterfly decline later in this paper.    As part of the cryptochrome  system insect/butterfly eyes are tuned to blue light but specifically polarised.  As early as 1965  von Frisch (1965), demonstrated that other insects (honeybees (Apis mellifera)) respond to skylight polarization and use it for navigation. Not only do butterflies produce polarisation sensitive colour in their wings ( ref) they also feed and navigate and select mates accordingly (refs). In recent months I have noticed urban and garden butterflies in Gwynedd behaving strangely, seemingly unable to orient towards the sun or to settle on flowers properly.  I thus wondered if something is effecting their polarisation detection system.    

 

 

Polarization-sensitive colour originates from polarization-dependent reflection or transmission, exhibiting abundant light information, including intensity, spectral distribution, and polarization. Such is seen with butterfly wings. Light reflected from waxy plant surfaces is partially linearly polarized, see Wehner and Bernard (1993).            A wide range of butterflies are physiologically sensitive to polarized light, but the origins of polarized signal have not been fully understood. Zhang et al (REF)  have  systematically investigated the colourful scales of six species of butterfly to reveal the physical origins of polarization-sensitive colour. Microscopic optical images under crossed polarisers exhibit their polarization-sensitive characteristic, and micro-structural characterizations clarify their structural commonality. In the case of the structural scales that have deep ridges, the polarization-sensitive colour related with scale azimuth is remarkable. Periodic ridges lead to the anisotropic effective refractive indices in the parallel and perpendicular grating orientations, which achieves form-birefringence, resulting in the phase difference of two different component polarized lights. Simulated results show that ridge structures with reflecting elements reflect and rotate the incident p-polarized light into s-polarized light. The dimensional parameters and shapes of grating greatly affect the polarization conversion process, and the triangular deep grating extends the outstanding polarization conversion effect from the sub-wavelength period to the period comparable to visible light wavelength. The parameters of ridge structures in butterfly scales have been optimized to fulfill the polarization-dependent reflection for secret communication. The structural and physical origin of polarization conversion provides a more comprehensive perspective on the creation of polarization-sensitive colour in butterfly wing scales.

 

 

Butterflies of the genus Papilio have polarisation-sensitive photoreceptors in all regions of the eye, and different spectral types of receptor are sensitive to different e-vector orientations. Zhang studied the consequences of this eye design for colour vision in behavioural tests and found that Papilio spp. see false colours due to the polarisation of light. They discriminate between vertically and horizontally polarised light of the same colour in the contexts of oviposition and feeding. The discrimination depends on the spectral composition of the stimuli. In the blue and probably in the green range, discrimination does not depend on intensity. However, colour discrimination is influenced by polarisation. Thus, colour and polarisation processing are not separated in the visual system of Papilio spp. From their  results, they  proposed hypotheses about which photoreceptors contribute to colour vision in Papilio spp. and what adaptation value such a system might have for the butterflies, reference at  http://onlinelibrary.wiley.com/doi/10.1002/2013GL058840/pdf.

 

Sweeney et al also comment on the use of polarisation by   Heliconius butterflies.

 

Holger G. Krapp Department of Bioengineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK has  further discussed how Insects Find Their Way by Watching the Sky and the polarised light it produces. 

 

 

It seems to me that anything which disturbs sky colour and/or skylight polarisation will impact heavily on pollinators such as bees or butterflies.   

 

 

The dominant backscattered electric field from the clear-sky Earth-atmosphere system is nearly parallel to the Earth surface. However, when clouds are present, this electric field can rotate significantly away from the parallel direction. Model results demonstrate that this polarization feature can even be used to detect super-thin cirrus clouds having an optical depth of only ~0.06 and super-thin liquid water clouds having an optical depth of only ~0.01. Such clouds are too thin to be sensed using any current passive satellite instruments, see Sun et al 2014,  http://onlinelibrary.wiley.com/doi/10.1002/2013GL058840/full yet at ground level and at some solar zenith angles they may completely rotate  the said field from parallel to perpendicular.    I postulate that this would severely disorient pollinators such as bees and butterflies.    

 

As more and more air travel takes place, I would expect more and more white skies and cirrus (aviaticus) cloud. I would thus expect more and more polarisation disturbance to the blue sky scenario and more and more confusion for insects such as butterflies.

 

To test this hypothesis, I plotted the percentage of butterfly population since 1990  against revenue passenger kilometres ( an indicator of amount of air travel ) in the same period, see below.   As air travel has intensified and as modern aero-engines and fuels have evolved, more and more aircraft contrails spread out and persist across the skies as a cirrus type haze.  

 

 

A very strong correlation is found R=.86.  This also exceeds temperature and chemical pollution effects. 

 

In North Wales  we have to date in 2016 had a warm but very cloudy summer with   clear days literally countable on one hand and even on dry days the sun often does not break through  the cirrus haze until mid -afternoon. I would expect this too may disrupt insect and even human  circadian rhythms.   Neonics (apparently?)  remain banned  but urban butterflies, particularly the Small Tortoiseshell  and Peacock  species appear all but extinct.  Both these species were present as normal in March and April of 2016.   Richard (Rik) Fox of Butterfly             Conservation also comments on the 2016 UK weather in general as having  a cold, late spring followed by a warm but extremely wet and sunless summer.  The results of this present study seem to indicate that it is not cloud or lack of sunshine per se which is contributing to butterfly decline but more likely it is specific types of cloud and light polarisation scenario brought on by the almost exponentially increasing amounts of air travel persisting at present.

 

Further discussion of white skies effect

A test of the white skies hypothesis would be to evaluate butterfly populations underneath air corridors as compared with those well way.  There is already considerable support for the idea.

 

Butterfly Conservation vice-president is Mr Chris  Packham.  Mr Packham has recently stated that butterflies in England are declining while those in Scotland show no long-term trend. He has blamed this on climate change.   

 

It seems to me that air traffic density offers a far better explanation.  Below is a typical flight radar image of air traffic over Britain and Europe.

 

 

I note that Scotland is one of the least overflown areas and has least butterfly decline.  

 

 

 

 On German Butterfly decline Jan Christian Habel, one of the study researchers from TUM's Terrestrial Ecology Research Group.

Despite climate warming, thermophilic species - those that like warm and dry conditions - also appear to be in decline.

 

Nitrogen input to agricultural land has been blamed for promoting too much plant growth and too much shade close to ground.

 

Light polarisation effects due to aircraft are of course not on the agenda.   But reference to the flight radar image shows Germany to be one of the most heavily overflown parts of Europe.

 

 

5.      The Radio Frequency  (RF) Environment

I have advanced a hypothesis elsewhere ( reference)  as to why butterflies may be impacted by 4G mobile communications systems.  

 

It would be interesting to see if any stepwise declines can be seen in the Defra data corresponding with the dates of introduction of any other  RF technologies.  I predict stepwise, rather than permanent declines because adaptation happens faster in organisms with short life cycles. For example, Sawicki and Denholm (1984) have discussed insect adaption to various insecticides. 

 

Butterfly declines have actually been recorded since the 1970's and this period does coincide with the introduction of UHF TV broadcasting.

 

The Defra data for recent declines is shown below.

 

Image result for plot of butterfly decline europe

I have previously suggested that from their body sizes most butterflies would act as dielectric resonators   at frequencies from about 600-1000MHz.

 

 

2G mobile telephony was introduced in Britain in 1993 and a step wise decline of 20% can be seen in all butterfly populations at that time.  Similar changes are seen in the EU butterfly data where mobile telephony followed a similar historic progression.    Not all EU countries have the same spring and summer weather conditions as Britain so this points to butterfly decline as involving far more complex factor(s) than   previously suggested by biologists and conservationists.

 

DAB was introduced in 1999.  Because of the much longer wavelength I would not expect to see an effect and as predicted no such effect is noted.

 

 

3G technology was introduced in 2001. 3G operates predominantly at 2100 MHz and so I would not expect such a pronounced effect, but there may be some effect as individual parts of a butterfly's body might resonate.  A 10% stepwise decline is noted in 2001.

 

DVB terrestrial TV was introduced mainly between 2010 -2012 in Britain and we see a corresponding 30% decline.  One should perhaps not read too much into this however, as firstly these frequencies were already in use for analogue TV and secondly the summers of 2011 and 2012 were both significantly colder than the long term average.  

 

As expected the effects of RF on butterfly populations appear to have been transient.  I postulate that RF interferes bi-modally with the cryptochrome system.   Firstly, rotating fields and these are expected to be far more of a problem with 4G but data is not yet fully available. 

 

Secondly, I would imagine RF may interfere with the insect immune response in a way not unlike that proposed by Lauer (ref|) for human carcinogenesis. For instance, it is known that   the Immune response in insects and mammals has certain common origins. Insects have a highly efficient immune system. In response to a bacterial attack, their fat body (the equivalent of the liver in mammals) synthesizes a whole range of peptides with an antibacterial and antifungal effect. For several years now the "Immune response and development in insects" Laboratory (CNRS, Strasbourg) led by Jules Hoffmann, has been studying the mechanisms that control the antimicrobial response in insects, using the Drosophila as their model. Thanks to a genetic approach developed by Bruno Lemaitre, several different control pathways (or "cascades") governing the expression of the genes that code for antimicrobial peptides have been identified. This researcher showed that one of these cascades, the Toll pathway, is structurally and functionally similar to a specific pathway in mammals responsible for the expression of the genes involved in the acute phase immune response. This research demonstrates that the cascade involved in the immune response must have appeared early on in the evolution of eucaryotes. It also illustrates the striking similarities between the antimicrobial response in insects and the innate, non-adaptive response in mammals 

1.      In mammals, the acute phase response refers to the changes in the bloodstream during primary response to infection. In this response, the blood cells and liver produce a whole range of proteins.
2. A mammal's primary response to infection depends on innate immunity, which is based on a variety of mechanisms that recognize and respond to the presence of a pathogen (including, among other things, the acute phase response). This response is followed by the adaptive response mediated through clonal selection of specific lymphocytes, which provides long-term protection against a given pathogen.

 

 

The somewhat surprising and almost counter-intuitive predictive upshot of changes in the RF environment are transitory. According to Lauer, for carcinogenesis in humans this is connected with the way in which the body and thymus in particular manufactures and stores different populations of T-cells.  As far as is presently Insects only have an innate immune system, maybe the above finding calls this into question?

 

 

 

Conclusions and further work

 

UK butterflies appear to be in a state of severe decline for a number of reasons.   This work finds that the strongest reason for their decline (73% of current rates) is the increasing prevalence of cloudy skies, especially aviation cirrus here in the UK.  Secondary reasons   include the neonicotinoid family of pesticides and the orgnophosphate herbicide glyphosphate.   Pesticides and herbicides possibly account   for the other 30% or so of current decline.  Transient declines (between 10-30 % of the 1990 total numbers) may have also occurred with the introduction of various new radio frequency communication and broadcasting technologies. 

 

The following further work is crucial to confirming the above hypotheses and preliminary findings:

1.      Establish butterfly numbers underneath UK air-lanes compared with numbers well separated from the same.   Flight radar evidence already suggests that most overflown areas are those with most butterfly decline.

2.      Establish decline ( if any)  due to 4G communications technology which  ought to show in 2012-2017 data. 

3.      Fight for moratorium on the use of 'round up' and similar glyphosphate weed killer products, to re-assess their effect.